Professional Analytical and Consulting Services

Basics of Gas Chromatography

PACS Course Number 45

9 AM to 5 PM

This 3-day course covers packed and capillary column gas chromatography (GC). It is designed for chemists, technicians and others who have minimum experience (0-3 years) with gas chromatography, or want to expand their knowledge base, or those interested in learning GC procedures. This course emphasizes: problem solving, practical day-to-day operation tips, minimum of theory, column and phase selection, detectors, applications, method development, quality control, and troubleshooting. All major GC detectors will be covered, the third day is devoted to the mass spectrometer detector and basic interpretation of data. PACS has four courses devoted to mass spectrometry. Also, GC applications will be discussed which are of interest to participants. Please note your experience and GC applications on the registration form. The course outline is below.

The instructor has well over thirty years of practical experiences using packed and capillary GC in many different applications. Dr. Nowicki's GC experiences include: environmental sciences, clinical, toxicology, forensics, legal and illegal drugs, natural products, metabolism, food sciences, R&D, industrial problem solving, biotechnology, and others. Dr. Nowicki has real-world experiences with all detectors discussed in this course, please see section IV below. This course emphasizes practical aspects of GC and lightly covers theory. The basics of GC method development and quality control and assurance for defensible GC analysis is covered.

Register by mailing a copy of the REGISTRATION FORM. Send it with a payment of $2395 to secure your attendance. Hotel information and a map will be sent to confirm your registration. Please list your special interest(s) on the registration form. Enrollment is limited to assure your maximum course benefits. On-site PACS courses are available (most PACS courses can be provided at your time and place). Telephone Henry Nowicki or Barb Sherman at 1.724.457.6576 if you need further information. PACS also provides laboratory testing and consulting services.



Basics of Gas Chromatography

Course Instructor: Dr. Henry Nowicki

Course Outline
I. INTRODUCTION
History, definitions, applications/purpose
Requirements, limitations, GC components/practical theory and practices
II. INJECTION SYSTEMS
Syringe types/methods, septa sweep
Packed and capillary, on-column, split/splitless injections
New techniques and applications, solvent effects
Background, maintenance/trouble-shooting strategies
III. COLUMN SYSTEMS
Packed and capillary
Performance evaluations, how they are made
Liquid phases/selection/temperature limits
Solid supports
Adsorbents, molecular sieves
Isothermal/temperature programming
Compatibility, practical operating tips
Installation of columns, trouble-shooting, gas flow adjustments
Linear velocity thru capillary column
Operational tips
Maintenance and trouble-shooting
IV. DETECTOR SYSTEMS
FID, TCD, ECD, NPD, HEC, PID, AED, MS and MSD
Strengths and weakness of each detector
Third day of course covers MS detector and MS data interpretations
V. QUALITATIVE IDENTIFICATION
Retention time match with standard, derivatives
Relative retention times and absolute
Detectors in series/ratios, non-destructive/destructive
Dual column confirmations
VI. QUANTIFICATION
Peak height, area
Internal/external standards
LOD/LOQ/PQL
Signal-to-noise ratio, background, overlaps
Data systems
VII. QUALITY ASSURANCE
System check-out, training, documentation, log books, calibrations
Blanks, standards, reference materials and audits
VIII. SAMPLE HANDLING
Collection, preservation, preparation, storage
Solid phase extraction, Liquid-liquid phase extraction
Purge-and-trap, thermal desorption, headspace
Holding times/stability, method selection and development
IX. SUMMARY
Literature and other information sources
Open discussion of individual separation problemS and special interests

View the PACS Short Course Schedule

Course participants are encouraged to e-mail questions to Dr. Henry Nowicki at

Please NOTE: List your special interest on the registration form. We will try to accommodate your interest. PACS also provides internet consulting information at http://pacslabs.com

Training is an Investment in employee employer Future!

For more information please contact:

Henry Nowicki, Ph.D. / M.B.A
PACS Testing, Consulting and Training
409 Meade Drive, Coraopolis, PA 15108
Phone: 1. 724. 457. 6576 or 1. 800. 367. 2587
E-Mail:
PACS website: http://pacslabs.com


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